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. 2020 Jul 27;31(9):1825–1832. doi: 10.1021/jasms.0c00070

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Published by the American Chemical Society. All rights reserved.

This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License, which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.

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Three-dimensional longitudinal visualization of the plaque-specific lipids m/z 480.3 (LPE 18:0/LPC 16:0) and m/z 599.3 (LPI 18:0) as detected by 3D MALDI mass spectrometry imaging in an aortic root of an apoe^–/– mouse (a). Spatial correlation analysis was performed for m/z 480.3 (LPE 18:0/LPC 16:0) on the annotated plaque area and for m/z 599.3 (LPI 18:0) on the annotated necrotic core area, respectively, on a section-by-section basis. This allows showing the variation of the correlation of two lipids to the respective annotations along the z-direction of the plaque (b). The blood flow direction is from left to right.