. 2020 Aug 15;7:1046–1056. doi: 10.1016/j.toxrep.2020.08.002

Table 2.

miRNAs in the placenta that are responsive to exposure to environmental toxicants.

Extrinsic Factors	Target Tissue(s)	Sample Size (N)	# of miRNAs Measured	Differentially Expressed miRNAs	Methods			Author (year)
Extrinsic Factors	Target Tissue(s)	Sample Size (N)	# of miRNAs Measured	Differentially Expressed miRNAs	Extraction	miRNA quantification	Normalization	Author (year)
Toxic Metals	Placenta, JEG-3 cells	N = 32	861	miR-26a, miR-155	AllPrep DNA/RNA/miRNA Univerisal Kit (Qiagen)	Human miRNA Oligo microarray (Agilent); qRT-PCR	miRNA U6	(Brooks, Martin et al. 2016)
Phthalates	Placenta	N = 179	29	miR-185, miR-142−3p, miR-15a-5p	mirVANA RNA Isolation Kit (Ambion)	PCR array (Qiagen); qRT-PCR	SNORD61, SNORD95	(LaRocca, Binder et al. 2016)
Phenols	Placenta	N = 80	1349	miR-146a^e	TRIzol Reagent (Invitrogen)	Human miRNA Microarray (Agilent); qRT-PCR	RNU6B	(De Felice, Manfellotto et al. 2015)
Phenols	Placenta	N = 179	29	miR-185, miR-142−3p, miR-15a-5p	mirVANA RNA Isolation Kit (Ambion)	PCR array (Qiagen); qRT-PCR	SNORD61, SNORD95	(LaRocca, Binder et al. 2016)
Particulate Matter	Placenta	N = 210	6	miR-16^d, miR-20a, miR-21^c, miR-146a^e, miR-222^f	miRNeasy mini kit (Qiagen)	Taqman miRNA qRT-PCR assay	RNU6	(Tsamou, Vrijens et al. 2018)
Particulate Matter	Placenta	N = 25	4	miR-16^d, miR-21^c, miR-146a^e	miRVANA miRNA Isolation Kit (Ambion)	Taqman miRNA qRT-PCR Assay	RNU44	(Maccani, Avissar-Whiting et al. 2010)

Reproduced miRNAs across studies: ^c miR-21, ^d miR-16, ^e miR-146a.