Skip to main content
. Author manuscript; available in PMC: 2021 Jul 2.
Published in final edited form as: Mol Cell. 2020 Jun 12;79(1):68–83.e7. doi: 10.1016/j.molcel.2020.05.029

Figure 3. HXMS identifies regiospecific conformational changes upon conversion of BAXM to BAXO.

Figure 3.

(A-B) HXMS profiles of BAXM and BAXO as measured over time in the presence of liposomes. Data are representative of two independent biological replicates.

(C) Deuterium difference plot showing the relative deuterium incorporation of BAXO minus that of BAXM, as measured over time with liposomes. Orange and green color scales indicate extent of conformational deprotection (increased exchange) and protection (decreased exchange), respectively. Data are representative of two independent biological replicates.

(D) Prominent regions of deprotection (N-terminus, α1, α7-α8 junction) and protection (α2, α4-α5 loop, proximal α5, distal α8, and α9) upon conversion of BAXM to BAXO are mapped onto BAXM (PDB: 1F16) according to the orange and green color scales (significance threshold, ± 1.0 Da).

See also Figure S5 and Table S1.