KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-BAX 2D2 | Santa Cruz | Cat# SC-20067; RRID: AB-626726 |
| Rabbit polyclonal anti-BAX N20 | Santa Cruz | Cat# sc-493; RRID: AB_2227995 |
| Sheep anti-mouse HRP | Bio-Rad | Cat# AAC10P; RRID: AB-321929 |
| Bacterial and Virus Strains | ||
| One Shot BL21(DE3) Competent Cells | Invitrogen | Cat# C600003 |
| LOBSTR BL21(DE3) Competent Cells | Kerafast | Cat# EC1001 |
| One Shot STBL3 Competent Cells | Invitrogen | Cat# C737303 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Rink Amide AM Resin LL 100-200 mesh | Millipore | Cat# 855120 |
| (S)-N-Fmoc-α-(4-pentenyl) alanine | Nagase & Co | Cat# 365023 |
| Grubbs Catalyst 1st Generation | Sigma-Aldrich | Cat# 579726 |
| 8-aminonapthalene-1,3,6-trisulfonic acid (ANTS) | Thermo Fisher Scientific | Cat# A350 |
| p-xylene-bis-pyridinium bromide (DPX) | Thermo Fisher Scientific | Cat# X1525 |
| Complete Protease Inhibitor Cocktail Tablets | Sigma-Aldrich | Cat# 16829800 |
| SimplyBlue SafeStain | Thermo Fisher Scientific | Cat# LC6065 |
| NativePAGE Sample Buffer (4X) | Thermo Fisher Scientific | Cat# BN2003 |
| NativePAGE Cathode Buffer Additive (20X) | Thermo Fisher Scientific | Cat# BN2002 |
| NativePAGE 5% G-250 Sample Additive | Thermo Fisher Scientific | Cat# BN2004 |
| NativePAGE Running Buffer (20X) | Thermo Fisher Scientific | Cat# BN2001 |
| Native Mark Unstained Protein Standard | Thermo Fisher Scientific | Cat# LC0725 |
| NativePAGE 4-16% Bis-Tris Protein Gels, 1.0 mM, 10 well | Thermo Fisher Scientific | Cat# BN1002BOX |
| Chitin bead resin | New England Biolabs | Cat# S6651S |
| SeeBlue Plus2 Pre-stained Protein Standard | Invitrogen | Cat# LC5925 |
| Sepharose CL-2B | GE Healthcare Life Sciences | Cat# 17014001 |
| Agilent C18 pOmix tips | Agilent | Cat# A57003 |
| EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) | ThermoFisher Scientific | Cat# A35391 |
| Ethylenediamine | Sigma Aldrich | Cat# 26266 |
| 2,2’-(ethylenedioxy)bis(ethylamine) | Sigma Aldrich | Cat# 385506 |
| Sulfo-NHS (N-hydroxysulfo Succinimide) | ThermoFisher Scientific | Cat# A39269 |
| DSSO (Disuccinimidyl Sulfoxide) | ThermoFisher Scientific | Cat# A33545 |
| 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine | Avanti | Cat# 850457C |
| 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine | Avanti | Cat# 850757C |
| L-α-phosphatidylinositol (Liver, Bovine) (sodium salt) | Avanti | Cat# 840042C |
| 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (sodium salt) | Avanti | Cat# 840035C |
| Cardiolipin (Heart, Bovine) (sodium salt) | Avanti | Cat# 840012C |
| Fos-choline 12 (Anagrade) | Anatrace | Cat# F308 |
| Detergent Screening Set Classic (n-Octyl-beta-D-glucopyranoside, N,N-Dimethyldodecylamine N-oxide, n-Decyl-beta-maltoside, n-Dodecyl-beta-maltoside, and n-Octyl-1-beta-D-thiglucopyranoside) |
Cube Biotechnology | Cat# 16092 |
| 3-((3-cholamidopropyl)dimethyl ammonio-1-propanesulfonate (CHAPS) | Thermo Fisher Scientific | Cat# 28300 |
| Factor XIII Protease from Aspergillus saitoi | Sigma Aldrich | Cat# P2143 |
| Pepsin from porcine gastric mucosa | Sigma Aldrich | Cat# P6887 |
| S63845 | MedChemExpress | Cat# HY-100741 |
| ABT-737 | Cayman Chemical | Cat# 11501 |
| Lipofectamine 3000 | ThermoFisher Scienfici | Cat# L3000008 |
| BIM SAHBA2 | Walensky Lab | N/A |
| BAX α6 SAHB | Walensky Lab | N/A |
| BFL-1 α6 SAHB | Walensky Lab | N/A |
| Recombinant BAX WT | Walensky Lab | N/A |
| Recombinant BAX I133A | Walensky Lab | N/A |
| Recombinant BAX G108E | Walensky Lab | N/A |
| Recombinant BAX R134E | Walensky Lab | N/A |
| Recombinant BAX R134E/R145E | Walensky Lab | N/A |
| Recombinant BAXΔC (aa 1-168) | Walensky Lab | N/A |
| Recombinant BAXΔC (aa 1-168) R134E | Walensky Lab | N/A |
| Recombinant BCL-w WT | Walensky Lab | N/A |
| Recombinant BFL-1ΔC (1-151) | Walensky Lab | N/A |
| Critical Commercial Assays | ||
| Q5 Site Directed Mutagenesis Kit | New England Biolabs | Cat# E0554S |
| CellTiter-Glo® Luminescent Cell Viability Assay | Promega | Cat# G7571 |
| Pierce BCA Protein Assay Kit | ThermoFisher Scientific | Cat# 23225 |
| Rat/Mouse Cytochrome c Quantikine ELISA Kit | R&D Systems | Cat# MCTC0 |
| Quantitative Colorimetric Peptide Assay | Pierce | Cat# 23275 |
| Experimental Models: Cell Lines | ||
| Bax−/−Bak−/− SV40 MEF | ATCC | Cat# CRL-2913 |
| Bax−/−Bak−/− SV40 MEF + BAX WT | Walensky Lab | N/A |
| Bax−/−Bak−/− SV40 MEF + BAX R134E/R145E | Walensky Lab | N/A |
| 293GPG Retroviral Packaging Line | Cantor Lab | N/A |
| Recombinant DNA | ||
| Plasmid PTYB1 | New England Biolabs | Cat# E6901 |
| Plasmid PTYB1_BAX_WT | Walensky Lab | N/A |
| Plasmid PTYB1_BAX_R134E | Walensky Lab | N/A |
| Plasmid PTYB1_BAX_R134E_R145E | Walensky Lab | N/A |
| Plasmid PTYB1_BAX_I133A | Walensky Lab | N/A |
| Plasmid PTYB1_BAX_G108E | Walensky Lab | N/A |
| Plasmid PTYB1_BAXΔC (aa 1-168) | Walensky Lab | N/A |
| Plasmid PTYB1_BAXΔC (aa 1-168) R134E | Walensky Lab | N/A |
| Plasmid PTYB1_BCL-w | Walensky Lab | N/A |
| Plasmid pet17b_BFL-1ΔC (aa 1-151) | Walensky Lab | N/A |
| Plasmid pMIG_BAX_WT | Genewiz | N/A |
| Plasmid pMIG | Addgene | Cat# 52107 |
| Plasmid pMIG_BAX_R134E_R145E | Walensky Lab | N/A |
| Software and Algorithms | ||
| Prism | Graphpad Software Inc. | https://www.graphpad.com/scientific-software/prism/ |
| Pymol | The PyMol Molecular Graphics System, Version 1.7.4.0 | Schrodinger, LLC |
| Chimera | UCSF | https://www.cgl.ucsf.edu/chimera/ |
| ProteinLynx Global Server (PLGS) 3.0.1 | Waters Corporation | www.waters.com/waters/en_US/ProteinLynx-Global-SERVER-(PLGS)/nav.htm?cid=513821&locale=en_US |
| DynamX 3.0 | Waters Corporation | www.waters.com/waters/library.htm?cid=511436&lid=134832928&locale=en_US |
| Proteome Discoverer 2.2 | Thermo Scientific | Thermo Scientific |
| XlinkX | Liu et al, 2015 | https://www.hecklab.com/software/xlinkx/ |
| Perculator | The et al, 2016 | http://percolator.ms/ |
| MatLab 2018 | Math Works | https://www.mathworks.com/products/matlab.html |
| GNOM | Svergun, 1992 | https://www.embl-hamburg.de/biosaxs/gnom.html |
| DAMMIN | Svergun, 1999 | https://www.embl-hamburg.de/biosaxs/dammin.html |
| Relion 2.0 | Scheres, 2012 | https://www3.mrc-lmb.cam.ac.uk/relion/index.php?title=Main_Page |
| Mathematica 12.0 | Wolfram | https://www.wolfram.com/mathematica/ |
| Other | ||
| Superdex 75 10/300 GL size exclusion column | GE Healthcare Life Sciences | Cat# 29148721 |
| Superose 6 Increase 10/300 GL size exclusion column | GE Healthcare Life Sciences | Cat# 29091596 |