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. Author manuscript; available in PMC: 2021 Aug 20.
Published in final edited form as: Cell Chem Biol. 2020 Aug 13;27(8):904–920. doi: 10.1016/j.chembiol.2020.07.022

Figure 4. Multicolor imaging with engineered BRET reporters.

Figure 4.

(A) A variety of luciferase-fluorescent protein (FP) fusions have been generated. Resonance energy transfer can provide a range of bioluminescent colors. (B) Example BRET reporters. These constructs comprise luciferase donors with either fluorescent protein or small molecule acceptors. (C) A panel of enhanced Nano- lanterns was expressed in HeLa cells, enabling real-time BRET imaging of cellular organelles. Reprinted with permission from Suzuki et al., 2016.