Figure 2.

The fractional contribution (FC) of GABA_AR subunits in human oligodendrocyte progenitor cells (OPCs). Normalized gene expression levels for all 19 GABA_AR subunits expressed as a percentage (mean ± S.E.M.) of the total available pool of mRNA for GABA_ARs in OPCs (PDGFRα⁺ cells) from human brains, estimated from publicly available datasets (Hodge et al., 2019; https://celltypes.brain-map.org/rnaseq). The single-nucleus analysis used normalized RNA-Seq datasets from the middle temporal gyrus isolated from six subjects with no known neuropsychiatry or neuropathological history (three males and three females; 35–66 years old). Gene expression level in each dataset was transformed into FC (Sequeira et al., 2019). FC is defined as the percentage of the expression level of each subunit gene (signaled in the “y” axis) to the sum of the 19 genes for GABA_ARs subunits within each human/cell. Detailed demographic characteristics, as well as technical white papers for data processing and quality control, can be downloaded from the same site. Confirmatory analysis of OPC markers enrichment and lack of neuronal markers were performed for all datasets.