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. 2020 Aug 27;2(2):fcaa105. doi: 10.1093/braincomms/fcaa105

Figure 8.

Figure 8

Effect of modulation of the cyclic-AMP/protein kinase and calcium-dependent pathways on striatal Gdnf gene expression under ex vivo and in vivo conditions. Vertical bar graphs indicate Gdnf mRNA level (measured by qPCR with Hmbs or Actb genes as internal reference) in the ST after 5 h incubation under ex vivo conditions, or 4 h after stereotaxic intrastriatal administration (in vivo). (A) Ex vivo stimulation of PKA by dibutyryl-cAMP (dbcAMP; 1 mM) versus control (CTL). (B) Ex vivo stimulation of AC by FSK (50µM) versus control (DMSO vehicle) (C) Ex vivo stimulation of PKA by dbcAMP (1 mM) and effect of 666-15 (10 µM), a selective CREB inhibitor. (D) Ex vivo stimulation of AC by FSK 50µM and effect of 10 µM of 666-15. (E) Ex vivo stimulation of PKA by dbcAMP (1 mM) and effect of CdCl2 (200 µM), a blocker of voltage-gated calcium channel. (F) Ex vivo stimulation of AC by FSK (50µM) and effect of CdCl2 (200 µM). (G) In vivo stimulation of PKA with intrastriatal administration of 49 µg dbcAMP, with controls receiving PBS. (H) In vivo stimulation of AC with intrastriatal administration of 8.2 µg FSK, with controls receiving DMSO. The number of experimental replicates (N) is indicated above the bar graphs. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, two-tailed Student’s t-test or one-way ANOVA with Tukey’s multiple comparison test.