Figure 6.

Protein expression of immunoregulatory genes in Tregs. Using flow cytometer, protein expression of immunoregulatory genes (CD73, PD1, IL13 and GZMB) were analysed in baseline and corresponding ex vivo expanded Tregs of Alzheimer patients (n = 5) and HCs (n = 5). Increase in the percentage of (A) CD73⁺, (B) PD1⁺ and (C) IL13⁺ Tregs (% of total CD4⁺CD25^high Tregs) were noted following ex vivo expansion in both Alzheimer patients and HCs. The change in the percentage of (D) GZMB⁺Tregs following expansion were not statistically significant. Mean fluorescence intensities (MFIs) of (E) CD73, (F) PD1 and (H) GZMB were amplified in expanded Tregs. The increase in G IL13 MFI in Tregs following expansion was not statistically significant. P-values are *P < 0.05, **P < 0.01 and ***P < 0.001.