Fig. 5.

Effect of oxidative stress on cofilin aggregation imaged by Atomic Force Microscopy. Morphological AFM images of the aggregates formed after 3 days on mica surfaces treated with Mg(II) by cofilin-2: WT, WT exposed 2 h to 50 μM H₂O₂, WT exposed 2 h to 50 μM H₂O₂ and then treated with 10 μM sulfide ion, S3D, S3D exposed 2 h to 50 μM H₂O₂ and S3D exposed 2 h to 50 μM H₂O₂ and then treated with 10 μM sulfide ion after three days. Protein concentration about 5 μM.