Figure 2.

SPRY4-IT1 depletion impaired the growth and glycolysis of CRC cells in vitro. (A). Quantitative PCR assays showed the expression of SPRY4-IT1 after the transfection of the indicated plasmids. (B). MTT assays exhibited the change of OD₄₉₀ levels upon the transfection of the indicated plasmids. (C). Colony formation assays were performed, and the colony number was quantified upon the transfection of the indicated plasmids in SW480 cells. (D). The levels of glucose uptake, lactate production, and cellular ATP levels were detected in SW480 cells upon the transfection of the indicated plasmids in SW480 cells. Results are presented as mean ± SEM, **p < 0.01.