Table 2.
Fatty acid profiles of experimental diets as determined by gas-liquid chromatography.
| Basal diet | Experimental diets | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Study 136 | Study 237 | Study 338 | |||||||||
| HF AIN-93G | AIN-93G | MTWD | MTWD ↓SF.ω-6 | ||||||||
| DHA (en%) | 0% | 0.96% | 2.40% | 0% | 0.87% | 2.62% | 0% | 2.63% | 0% | 2.63% | |
| DHA (%w/w) | 0% | 0.40% | 1.20% | 0% | 0.40% | 1% | 0% | 1% | 0% | 1% | |
| Common name | Chemical formula | (% of total fatty acids) | |||||||||
| Lauric | C12:0 | 0.07 ± 0.00 | 0.30 ± 0.06 | 0.75 ± 0.03 | 0.04 ± 0.00 | 0.62 ± 0.00 | 1.42 ± 0.07 | 0.65 ± 0.01 | 1.49 ± 0.03 | 0.14 ± 0.01 | 0.83 ± 0.01 |
| Myristic | C14:0 | 0.18 ± 0.01 | 0.97 ± 0.21 | 2.57 ± 0.11 | 0.16 ± 0.01 | 1.79 ± 0.02 | 4.15 ± 0.21 | 2.87 ± 0.06 | 5.54 ± 0.05 | 0.53 ± 0.01 | 2.51 ± 0.02 |
| Pentadecanoic | C15:0 | 0.03 ± 0.00 | 0.03 ± 0.00 | 0.03 ± 0.00 | 0.03 ± 0.00 | 0.02 ± 0.00 | 0.03 ± 0.00 | 0.29 ± 0.00 | 0.30 ± 0.00 | 0.07 ± 0.00 | 0.06 ± 0.00 |
| Palmitic | C16:0 | 8.31 ± 0.12 | 8.94 ± 0.33 | 10.05 ± 0.11 | 5.64 ± 0.04 | 6.82 ± 0.02 | 7.79 ± 0.02 | 22.24 ± 0.16 | 22.24 ± 0.05 | 10.87 ± 0.06 | 11.00 ± 0.01 |
| Palmitoleic | C16:1ω7 | 0.09 ± 0.00 | 0.18 ± 0.02 | 0.34 ± 0.01 | 0.08 ± 0.00 | 0.41 ± 0.00 | 0.86 ± 0.03 | 0.13 ± 0.01 | 0.10 ± 0.00 | 0.13 ± 0.01 | 0.12 ± 0.01 |
| Strearic | C18:0 | 2.61 ± 0.04 | 2.58 ± 0.07 | 2.47 ± 0.03 | 0.03 ± 0.00 | 0.04 ± 0.00 | 0.04 ± 0.00 | 8.49± 0.04 | 8.26 ± 0.03 | 3.61 ± 0.03 | 3.37 ± 0.01 |
| Oleic | C18:1ω9 | 46.36 ± 0.87 | 42.55 ± 2.21 | 35.56 ± 1.05 | 71.08 ± 0.13 | 62.40 ± 0.08 | 53.26 ± 0.50 | 39.19 ± 0.38 | 29.00 ± 0.08 | 72.63 ± 0.08 | 64.41 ± 0.03 |
| Linoleic | C18:2ω6 | 36.55 ± 0.70 | 36.09 ± 1.14 | 34.51 ± 0.70 | 19.17 ± 0.10 | 18.94 ± 0.01 | 15.08 ± 0.79 | 21.07 ± 0.21 | 20.62 ± 0.18 | 8.57 ± 0.04 | 7.82 ± 0.04 |
| Arachidic | C20:0 | 0.32 ± 0.01 | 0.31 ± 0.01 | 0.28 ± 0.00 | 0.31 ± 0.00 | 0.28 ± 0.00 | 0.24 ± 0.00 | 0.21 ± 0.03 | 0.17 ± 0.01 | 0.29 ± 0.00 | 0.28 ± 0.01 |
| Alpha-linolenic | C18:3ω3 | 3.15 ± 0.08 | 3.25 ± 0.08 | 3.26 ± 0.09 | 0.36 ± 0.01 | 0.37 ± 0.00 | 0.32 ± 0.01 | 1.90 ± 0.03 | 1.81 ± 0.04 | 0.93 ± 0.01 | 0.87 ± 0.02 |
| Behenic | C22:0 | 0.28 ± 0.01 | 0.28 ± 0.01 | 0.28 ± 0.01 | 0.22 ± 0.00 | 0.21 ± 0.00 | 0.20 ± 0.00 | 0.10 ± 0.01 | 0.11 ± 0.01 | 0.10 ± 0.01 | 0.11 ± 0.00 |
| Lignoceric | C24:0 | 0.13 ± 0.01 | 0.12 ± 0.01 | 0.12 ± 0.00 | 0.14 ± 0.00 | 0.13 ± 0.00 | 0.12 ± 0.00 | 0.04 ± 0.01 | 0.05 ± 0.01 | 0.03 ± 0.00 | 0.05 ± 0.00 |
| Eicosapentaenoic | C20:5ω3 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.01 ± 0.00 | 0.00 ± 0.00 | 0.09 ± 0.00 | 0.20 ± 0.01 | 0.00 ± 0.00 | 0.13 ± 0.00 | 0.00 ± 0.00 | 0.10 ± 0.01 |
| Docosahexaenoic | C22:6ω3 | 0.00 ± 0.00 | 2.47 ± 0.74 | 7.74 ± 0.43 | 0.00 ± 0.00 | 5.40 ± 0.05 | 14.20 ± 0.65 | 0.00 ± 0.00 | 7.09 ± 0.20 | 0.00 ± 0.00 | 6.34 ± 0.08 |
| Total saturated fat | 12.1 ± 0.2 | 13.7 ± 0.7 | 16.8 ± 0.2 | 8.3 ± 0.1 | 11.5 ± 0.1 | 15.4 ± 0.1 | 35.28 ± 0.21 | 38.84 ± 0.05 | 15.80 ± 0.08 | 18.39 ± 0.02 | |
| Total MUFA | 47.8 ± 0.9 | 44.0 ± 2.2 | 37.1 ± 1.1 | 71.9 ± 0.1 | 63.5 ± 0.1 | 54.7 ± 0.5 | 41.74 ± 0.36 | 31.49 ± 0.10 | 74.70 ± 0.10 | 66.49 ± 0.01 | |
| Total ω-6 PUFA | 36.7 ± 0.7 | 36.1 ± 1.14 | 34.6 ± 0.7 | 19.2 ± 0.1 | 18.9 ± 0.1 | 15.1 ± 0.8 | 21.07 ± 0.21 | 20.62 ± 0.18 | 8.57 ± 0.04 | 7.82 ± 0.04 | |
| Total ω-3 PUFA | 3.2 ± 0.1 | 5.8 ± 0.9 | 11.1 ± 0.4 | 0.4 ± 0.00 | 5.9 ± 0.1 | 14.7 ± 0.1 | 1.90 ± 0.03 | 9.04 ± 0.19 | 0.93 ± 0.01 | 7.31 ± 0.05 | |
| ω-6:ω-3 ratio | 11.6 ± 0.3 | 6.4 ± 0.9 | 3.12 ± 0.13 | 52.8 ± 1.3 | 3.2 ± 0.0 | 1.0 ± 0.1 | 11.07 ± 0.10 | 2.28 ± 0.07 | 9.24 ± 0.13 | 1.12 ± 0.01 | |
MUFA, monounsaturated fatty acid; PUFA, polyunsaturated fatty acid.