KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-IL3R antibody | R&D Systems | Cat# AF549; RRID:AB_355432 |
| TCR-β (H57–597)-FITC | eBioscience | Cat# 11–5961-82; RRID:AB_465323 |
| F4/80 (BM8)-FITC | eBioscience | Cat# 11–4801-82; RRID:AB_465323 |
| CD2 (RM2–5)-FITC | eBioscience | Cat# 11–0021-82; RRID:AB_464873 |
| CD3e (145–2C11)-FITC | eBioscience | Cat# 11–0031 −81; RRID:AB_464881 |
| CD4 (GK1.5)-FITC | eBioscience | Cat# 11–0041-82; RRID:AB_464892 |
| CD8b (Ly-3)-FITC | BioLegend | Cat# 126606; RRID:AB_961295 |
| CD19 (eBiol D3)-FITC | eBioscience | Cat# 11–0193-82; RRID:AB_657666 |
| CD45R (B220, RA3–6B2)-FITC | eBioscience | Cat# 14–0452-82; RRID:AB_467254 |
| Gr-1 (Ly6G, RB6–8C5)-FITC | eBioscience | Cat# 11–5931-82; RRID:AB_465314 |
| Cd11b (Mac1, M1/70)-FITC | eBioscience | Cat# 11–0112-41; RRID:AB_11042156 |
| Ter119 (Ly76)-FITC | eBioscience | Cat# 11–5921-82; RRID:AB_465311 |
| NK1.1 (Ly53, PK136)-FITC | eBioscience | Cat# 11–5941-82; RRID:AB_465318 |
| c-Kit (CD117, ACK2)-APCeFluor780 | eBioscience | Cat# 47–1172-82; RRID:AB_1582226 |
| Sea-1 -Pacific blue | Biolegend | Cat# 108119; RRID:AB_493274 |
| FcgRII/III-PE (CD16/32, 2.4G2) | Biolegend | Cat# 156606; RRID:AB_2800704 |
| CD34 (RAM34)-AlexaFluor 647 | Biolegend | Cat# 119314; RRID:AB_604089 |
| CD135 (Flt3, A2F10)-PE | Biolegend | Cat# 135305; RRID:AB_1877218 |
| CD150 (Slamfl, TC15–12F12.2)-PECy7 | Biolegend | Cat# 115913; RRID:AB_439796 |
| CD115 (AFS98)-APC | Biolegend | Cat# 135509; RRID:AB_2085222 |
| CD45 (30-F11)-APCCy7 | Biolegend | Cat# 103115; RRID:AB_312980 |
| Ly6C/G or Gr-1 (RB6–8C5)-PercPCy5.5 | BD Bioscience | Cat# 552093; RRID:AB_394334 |
| ABCA1 Antibody | Abeam | Cat# ab18180; RRID:AB_444302 |
| TET2 Antibody | Cell Signaling | Cat# 4501 OS; RRID:AB_2799277 |
| Phospho-Jak2 (Tyr1008) (D4A8) Rabbit mAb | Cell Signaling | Cat# 8082; RRID:AB_10949104 |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) | Cell Signaling | Cat# 4377; RRID:AB_331775 |
| Bacterial and Virus Strains | ||
| pLKO lentiviral | Genecust | N/A |
| Biological Samples | ||
| Peripheral blood | MSKCC | HSPCMSKCC06–107 |
| Bone marrow | MSKCC | HSPCMSKCC06–107 |
| Buccal swab | MSKCC | HSPCMSKCC06–107 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| poly:IC | Invivogen | tlrl-pic-5 |
| 5-fluorouracile | Sigma | F6627 |
| IL-6, Mouse | Milteny Biotech | 130–094-065 |
| IL-3 Culture Supplement, Mouse | Corning | 354040 |
| Stem Cell Factor, Mouse | Milteny Biotech | 130–094-079 |
| polybrene | Sigma | TR-1003 |
| 4% paraformaldehyde | Sigma | 1004968350 |
| LipofectAMINE 2000 | Invitrogen | 11668030 |
| GM- CSF | R&D Systems | 415-ML-005 |
| Methyl-β-cyclodextrin | Sigma | C4555 |
| Tempol | EMD Millipore | 581500, CAS 2226–96-2 |
| Thymidine, [Methyl-3H] | PerkinElmer | NET027W001 MC |
| Phorbol myristate acetate | Sigma | P8139 |
| Apolipoprotein A-l from human plasma | Sigma | A0722 |
| Bodipy-cholesterol probe | Life Technologies | D3922 |
| Fixation Buffer (BD Cytofix) | BD Bioscience | 554655 |
| Perm Buffer III | BD Bioscience | BD Bioscience |
| Cholera Toxin Subunit B, Alexa Fluor 594 conjugate | Invitrogen | C34777 |
| DAPI (4’,6-diamidino-2-phenylindole) | ThermoFisher Scientific | D1306 |
| Critical Commercial Assays | ||
| Total Cholesterol colorimetric kits | Wako Chemicals | 999–02601 |
| MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric) | Epigentek | P-1032–96 |
| Experimental Models: Cell Lines | ||
| HEK293 cells (human embryonic kidney) | ATCC | CRL-1573 |
| THP-1 monocytes (human acute monocytic leukemia cell line) | ATCC | TIB-202 |
| Experimental Models: Organisms/Strains | ||
| WT mice | Jackson Laboratory | SN000664 |
| Mx1-Cre mice (B6.Cg-Tg(Mx1-cre)1Cgn/J) | Jackson Laboratory | SN003556 |
| Abca1fl/fl mice (B6.129S6-Abca1tm1 Jp/J) | Jackson Laboratory | SN028266 |
| Tet2fl/fl (B6; 129S-Tet2tm 1.1 laai/J) | Jackson Laboratory | SN017573 |
| Human apoA-1 transgenic (B6.Tg(ApoA1)1Rub/J) | Jackson Laboratory | SN 001927 |
| Oligonucleotides | ||
| See Table S1 for genetic analysis of primary patient samples | N/A | N/A |
| $Software and Algorithms | ||
| FlowJo software | Tree Star | https://www.flowjo.com |
| Other | ||
| 4–20% Criterion Tris-HCI Protein Gel, 12+2 well, 45 μl | BioRad | 3450032 |
| Fetal Bovine Serum, Heat Inactivated | Fischer scientific | 12350273 |
| Penicillin-Streptomycin (10,000 U/mL) | ThermoFischer | 15140122 |
| RMP11640, no glutamine | ThermoFischer | 31870074 |
| L-Glutamine (200 mM) | ThermoFischer | 25030024 |