Figure 2.

TRPM-regulated trans-dedifferentiation of Schwann cells involves in MAPK signaling pathway via a p38-MAPK-independent manner. (A) Western blot analysis showed protein expression of phosphorylated c-Jun (p-c-Jun), phosphorylated extracellular signal-regulated kinase (pERK)-1/2 and phosphorylated p38MAPK (p-p38MAPK) (B) Relative intensities quantified the expression patterns of p-c-jun, p-ERK1/2 and p-p38MAPK in ex vivo trans-dedifferentiated Schwann cell (n = 9). (C) Sciatic nerve fibers were immunostained with anti-S100 (green) and p-ERK-1/2 (red). Scale bar = 50 μm. (D) Relative intensities indicated the effect of TRPM7 inhibition on ex vivo Schwann cell trans-dedifferentiation (n = 3).