KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Deposited data | ||
| Raw imaging and western data | This paper | https://data.mendeley.com/datasets/d532xvdrn2/draft?a=cdd41557-9d54-48fc-bc37-1e63b2be48bb |
| Antibodies | ||
| Rabbit polyclonal anti-ZGRF1 | This paper | Lot no. 28429 |
| Rat anti-tubulin | Abcam | Cat#ab6160; RRID:AB_305328 |
| Swine anti-rabbit | Dako | Cat#P0217; RRID:AB_2728719 |
| Rabbit anti-rat | Dako | Cat#P0450; RRID:AB_2630354 |
| Mouse anti-GFP | Roche Diagnostics | Cat#11814460001; RRID:AB_390913 |
| Rabbit anti-mouse | Dako | Cat#P0161; RRID:AB_2687969 |
| Mouse anti-FANCM | Novusbio | Cat#NBP2–50418; RRID:AB_2716711 |
| Rabbit anti-FANCJ | Novusbio | Cat#NB100–416; RRID:AB_2066307 |
| Rabbit anti-FANCD2 | Novusbio | Cat#NB100–182SS; RRID:AB_1108397 |
| Mouse anti-γH2AX | Millipore | Cat#05–636; RRID:AB_309864 |
| Alexa Fluor 568 goat anti-rabbit IgG | Life Technologies | Cat#A-11011; RRID:AB_143157 |
| Alexa Fluor 488 goat anti-mouse IgG | Life Technologies | Cat#A-21121; RRID:AB_2535764 |
| Rabbit anti-RAD51 | Bio Academia | Cat#70–001; RRID:AB_2177110 |
| Anti-GST-HRP | New England Biolabs | Cat#E2624S |
| Rabbit anti-human RAD51 | Santa Cruz Biotech | Cat#sc-8349; RRID:AB_2253533 |
| Anti-yeast Rad51 | Lab raised | N/A |
| Mouse anti-myc-HRP | Thermo Fisher Scientific | Cat#R951 −25; RRID:AB_2556561 |
| Mouse anti-FLAG-HRP | Sigma | Cat#A8592; RRID:AB_439702 |
| Rat anti-HA-HRP | Roche | Cat#12013819001; RRID:AB_390917 |
| Anti-human PCNA | Lab raised | N/A |
| Rabbit anti-human RPA70 | Abcam | Cat#ab79398; RRID:AB_1603759 |
| Rabbit anti-mouse IgG-HRP | Pierce | Cat#31450; RRID:AB_228427 |
| Goat anti-rabbit IgG-HRP | Sigma | Cat#A6154; RRID:AB_258284 |
| Goat anti-rat IgG-HRP | Santa Cruz Biotech | Cat#Sc-2032; RRID:AB_631755 |
| Bacterial and Virus Strains | ||
| E. coli strain DH5α | Lifetechnologies | Cat#18258012 |
| E. coli strain BL21(DE3) | New England Biolabs | Cat#C2527I |
| E. coli strain DH10Bac | Invitrogen | Cat#10361012 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Complete protease inhibitor cocktail | Roche | Cat#25735720 |
| Dynabeads Protein G | Life Technologies | Cat#10004D |
| GFP-Trap® M beads | Chromotek | Cat# gtm-20 |
| Mitomycin C | Sigma | Cat#M7949 |
| Talazoparib | SMS-Gruppen Denmark | Cat#S7048 |
| Hydroxyurea | Sigma | Cat#H8627 |
| KaryoMAX colcemid | Life Technologies | Cat#15212–012 |
| BrdU | Sigma | Cat#B5002 |
| Camptothecin | Merck | Cat#C9911 |
| Critical Commercial Assays | ||
| Guide-it sgRNA Screening Kit | Takara Bio | Cat#632639 |
| Factor Xa Cleavage Capture Kit | Merck | Cat#69037 |
| DPX Mountant for histology | Sigma | Cat#06522 |
| Duolink In Situ PLA Probe Anti-Rabbit PLUS | Sigma-Aldrich | Cat#DUO92002 |
| Duolink In Situ PLA Probe Anti-Mouse MINUS | Sigma-Aldrich | Cat#DUO92004 |
| Duolink In Situ Detection Reagents Orange | Sigma-Aldrich | Cat#DUO92007 |
| Experimental Models: Cell Lines | ||
| HCT116 | (Brattain et al., 1981) | N/A |
| U2OS | (Pontén and Saksela, 1967) | N/A |
| RPE-1 | (Jiang et al., 1999) | N/A |
| Oligonucleotides | ||
| Primers and oligonucleotides, see Table S3 | This paper | N/A |
| Recombinant DNA | ||
| pX458 | (Ran et al., 2013) | Addgene Plasmid #48138 |
| pBluescript SK+ | Fermentas GmbH, Germany | (Short et al., 1988) |
| pAB_KO1 | This paper | N/A |
| pAB_KO_rev | This paper | N/A |
| pAB_KO_rev3 | This paper | N/A |
| pX461 | (Ran et al., 2013) | Addgene Plasmid #48140 |
| pAB_2xYFP_A | This paper | N/A |
| pAB_2xYFP_B | This paper | N/A |
| pAK_ZGRF1–2xYFP | This paper | N/A |
| pCre-GFP | (Williams et al., 2015) | N/A |
| pKSV1 | This paper | N/A |
| pKSV2 | This paper | N/A |
| pKSV15 | (Motnenko et al., 2018) | N/A |
| pGEX-3X-ZGRF1-N | This paper | N/A |
| pCS2-mRFP | (Sartori et al., 2007) | N/A |
| pCBA-I-SceI | (Richardson et al., 1998) | N/A |
| Software and Algorithms | ||
| TIDE (https://tide.nki.nl) | N/A | (Brinkman et al., 2014) |
| FlowJo v10.4.1 | FlowJo, LLC | RRID:SCR_008520 |
| Volocity v5.4 | PerkinElmer | RRID:SCR_002668 |
| SoftWoRx 7.0.0 | Applied Precision | N/A |
| Prism 8 | GraphPad software | https://www.graphpad.com/scientific-software/prism/; RRID: SCR_000306 |