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. 2020 Jul 9;9(1):1580–1589. doi: 10.1080/22221751.2020.1787798

Figure 4.

Figure 4.

Production of vsRNAs in BHK cells and brain tissues of WT mice infected with SINV. (A- C) Size distribution of 18- to 28-nt SINV-derived small RNAs of both strands (A), of only genomic or antigenomic strand (B), and the canonical duplexes with 3′ 2-nt overhangs of 22-nt vsiRNAs (C) sequenced from BHK cells infected with SINV at 24 hpi. Reads are shown as per million mature miRNAs. 5′ terminal nucleotide of vsRNAs is indicated by colour. 1U % of 21- to 23-nt vsiRNA is given in parenthesis. The percentage of 22-nt SINV vsRNAs with 2-nt 3′ overhangs in the total 22-nt vsRNAs was shown in box. (D, E) Size distribution, canonical duplexes with 3′ 2-nt overhangs of SINV-derived small RNAs sequenced from SINV-infected BALB/c suckling mice by i.p. (D) and C57BL/6 adult mice by intracranial injection (E). (F) Size distribution of total sequenced vsRNAs in the libraries constructed from Brainstem, Cerebellum, Striatum, Hippocampus and Cerebral Cortex of SINV-infected BALB/c adult mice (from left to right).