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. Author manuscript; available in PMC: 2021 May 18.
Published in final edited form as: Methods Enzymol. 2020 May 18;640:1–35. doi: 10.1016/bs.mie.2020.04.001

Fig. 10.

Fig. 10

Changes in fluorescence of complete media seeded with different cell lines on a 96-well plate. Cells were treated with PS3 (10μM), IgG/DNP-beads (200,000/mL), DNP-beads alone (200,000/mL), or IgG alone (2μg/mL) for 4h (A) or 24h (B) at 37°C in a CO2 (5%) incubator. In (C), the myeloperoxidase inhibitor AZD5904 was additionally added to RAW 264.7 or J774A.1 cells with PS3 and IgG/DNP-beads (4h). Error bars represent SD (N ≥3). Statistical significance analyzed by one-way ANOVA (GraphPad Prism 8): ****, P <0.0001; ***; P <0.001; **, P <0.01. Samples were analyzed with a BMG LabTech Clariostar Plus plate reader (Ex. 480±7.5nm /Em. 520±7.5nm).