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. 2020 Aug 20;24(4):233–241. doi: 10.1080/19768354.2020.1808529

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Induction of NO and PGE₂ production in I6CA-treated RAW 264.7 cells. The cells were treated with I6CA or LPS for 24 h. The levels of NO (A) and PGE₂ (B) in culture media were measured. Data are presented as the means ± SD obtained from three independent experiments (^#p < 0.05 compared to the control group). The expressions of iNOS and COX-2 mRNA (C) and protein (D) were detected by RT-PCR and immunoblotting. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and actin were used as the internal controls for the RT-PCR and immunoblotting, respectively.