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. 2020 Aug 20;24(4):233–241. doi: 10.1080/19768354.2020.1808529

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Increased secretion and expression of cytokines by I6CA-treated RAW 264.7 cells. The cells were treated with I6CA or LPS for 24 h. (A-D) Cytokine concentrations were measured by ELISA kits. Data are presented as the means ± SD obtained from three independent experiments (^#p < 0.05 compared to the control group). The expressions of cytokines mRNA (E) and protein (F) were detected by RT-PCR and immunoblotting. GAPDH and actin were used as the internal controls for the RT-PCR and immunoblotting, respectively.