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. 2020 Aug 20;24(4):233–241. doi: 10.1080/19768354.2020.1808529

Figure 5.

Figure 5.

Induction of TLR4 and Myd88 by I6CA in RAW 264.7 cells. The cells were treated with I6CA or LPS for 24 h. (A) The levels of TLR4 and Myd88 protein were evaluated by Western blot analysis. Actin was used as an internal control. (B) Co-IP assay indicated that I6CA-induced TLR4 interacted with Myd88. IgG serves as a negative control of IP.