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. 2020 Jul 20;9(1):1664–1670. doi: 10.1080/22221751.2020.1791738

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — (A) Entry assay of pseudotyped virus. 50 μl pseudovirus was used to infect 2 × 10⁴ HEK293-ACE2, Huh7, Vero-E6 and HEK-293T cells, respectively. Luciferase activity was measured 48 h postinfection using the Promega kit. Triplicates were tested in each experiment. The average values and standard error bars are presented. The experiment was repeated three times with similar results obtained. (B) Comparison of pseudotyped neutralization assay with the live SARS-CoV-2 based MN assay. Log-transformed NAb titers (IC₅₀) are presented in the plot. Pearson correlation test results demonstrated a significant positive correlation (p < 0.0001) between two assays.