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. 2020 Aug 31;53(8):425–430. doi: 10.5483/BMBRep.2020.53.8.268

Fig. 4.

Fig. 4

Inhibition of CD44 decreases expressions of pGSK3β, nuclear β-catenin and active YAP-1 in TSG-6-treated pHSCs. (A) Immunoblot and cumulative densitometric analyses of inactive/p-GSK3β, GSK3β in whole lysate of vehicle-(vehicle) or TSG-6-treated pHSCs (TSG-6) trans-fected with scramble siRNA (scrRNA + TSG-6) or siRNA targeting CD44 (CD44 siRNA + TSG-6). pHSCs were treated with scrRNA or CD44 siRNA for 24 hours before TSG-6 treatment and then treated with vehicles or TSG-6 for 6 hours. (B) Immunoblot and cumulative densitometric analyses of active β-catenin and TCF4 in nuclear extracts of these cells. LAMIN B1 was used as an internal control for nuclear fraction. (C) Immunoblot and cumulative densi-tometric analyses of nuclear YAP-1 and TAZ in scrRNA or CD44 siRNA-transfected pHSCs treated with vehicle or TSG-6 for 24 hours. All data shown represent one of three experiments with si-milar results. Mean ± SD results are graphed (one-way ANOVA with Tukey corrections, $P < 0.05 vs. vehicle, *P < 0.05 vs. scrRNA + TSG-6).