Fig. 3.

Bee venom regulates the expression of cell-cycle inhibitors, apoptosis-related proteins, and mitogenic signaling-related proteins. (A) Western blot showing the time-dependent effects of BV on Rb, cyclin A, and cyclin B in Caski and HeLa cells treated for 0, 12, and 24 h with BV (5 µg/ml). (B) Expression of p53, p21, and p27 in Caski and HeLa cells treated with 5 µg/ml of BV was measured at 0, 12, and 24 h by Western blot. (C) Western blot of Caski and HeLa cells treated with 5 µg/ml of BV for 0, 12, and 24 h. Immunoblot analysis of Bax, Bcl-2, Bcl-XL, pro-caspase-3, pro-caspase-9, and cleaved PARP. (D) We measured protein levels of p-AKT, AKT, p-JNK, JNK, p-P38, P38, p-P44/42, and P44/42 by Western blot in Caski and HeLa cells treated with 5 µg/ml of BV for 0, 12, and 24 h. β-actin was used as a loading control. Data are presented as mean ± SD (n = 3). Significant differences are indicated with an asterisk (*P < 0.05, *P < 0.01), P values were calculated using the Student’s t test.