Fig. 1.

Improved transfection efficiency, prolonged sustainability, and increased cell viability by plasmids lacking CpG sequences. (A) GFP⁺ RAW 264.7 macrophages were analyzed by fluorescence microscopy two days after microporation. Scale bar = 100 µm. (B) GFP⁺ macro-phages were analyzed by flow cytometry. (C) The percentages of GFP⁺ macrophages were quantified for six days by flow cytometry. (D) Cell viability was measured using a Luna Dual fluorescence cell counter. Data represent the means ± standard deviation (SD), n = 3. Statistical significances were determined by two-way (C) or one-way (D) ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001.