KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-Vglutl polyclonal antibody | Synaptic Systems | Cat# 135303 |
| RRID: AB_887875 | ||
| Mouse anti-PSD95 antibody | NeuroMab (UC Davis) | Cat# 75-028 |
| RRID: AB_2307331 | ||
| Rabbit anti-Vgat polyclonal antibody | Synaptic Systems | Cat# 131002 |
| RRID: AB_887871 | ||
| Mouse anti-Gephyrin polyclonal antibody | Synaptic Systems | Cat# 147011 |
| RRID: AB_887717 | ||
| Goat anti-Rabbit IgG Alexa Fluor 488 | Thermofisher Scientific | Cat# A-11008 |
| RRID: AB_143165 | ||
| Goat anti-Mouse Alexa Fluor 647 | Thermofisher Scientific | Cat# A32728 |
| RRID: AB_2633277 | ||
| Bacterial and Virus Strains | ||
| pLenti- CAG- Flex-Wnt7b-IRES-GFP | This paper | N/A |
| pLenti-DlxI12b-Wnt7b-GFP | This Paper | N/A |
| pLenti-DlxI12b-GFP | This Paper | N/A |
| pLenti-CAG-Flex-IRES-GFP | This Paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Sucrose | Sigma Aldrich | Cat# S5016 |
| Sodium bicarbonate (NaHCO3) | Sigma Aldrich | Cat# S6014 |
| Glucose | Sigma Aldrich | Cat# G5767 |
| Magnesium sulfate (MgSO4) | Sigma Aldrich | Cat# 230391 |
| Sodium phosphate monobasic monohydrate (NaH2PO4) | Sigma Aldrich | Cat# P9638 |
| Potassium chloride (KCl) | Sigma Aldrich | Cat# P9333 |
| Calcium chloride dehydrate (CaCl2) | Sigma Aldrich | Cat# 223506 |
| Magnesium chloride dexahydrate (MgCl2) | Sigma Aldrich | Cat# M9272 |
| Potassium gluconate (KGluconate) | Sigma Aldrich | Cat# P1847 |
| HEPES | Sigma Aldrich | Cat# H3375 |
| EGTA | Sigma Aldrich | Cat# E4378 |
| Adenosine 5′-triphosphate magnesium salt (Mg-ATP) | Sigma Aldrich | Cat# A9187 |
| Guanosine 5′-triphosphate sodium salt hydrate (Na3GTP) | Sigma Aldrich | Cat# 51120 |
| Cesium Methanesulfonate | Sigma Aldrich | Cat# C1426 |
| Sodium Chloride (NaCl) | Sigma Aldrich | Cat# S9888 |
| QX314 chloride | Tocris | Cat# 2313 |
| ZD7288 | Tocris | Cat# 1000 |
| Critical Commercial Assays | ||
| Bioanalyzer High Sensitivity DNA Kit | Agilent | Cat# 5067-4626 |
| Bioanalyzer RNA 6000 Nano Kit | Agilent | Cat# 5067-1511 |
| Chromium i7 Multiplex Kit, | 10X Genomics | Cat# 120262 |
| Chromium Single Cell 3’ Chip Kit v2 | 10X Genomics | Cat# 120236 |
| Chromium Single Cell 3′ Library & Gel Bead Kit v2 | 10X Genomics | Cat# 120237 |
| Deposited Data | ||
| Tbr1 P5 scRNA-seq Raw and Analyzed Data | This paper | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE146298 |
| Experimental Models: Cell Lines | ||
| Mouse primary cortical culture | This paper | N/A |
| HEK293 cells | Thermofisher Scientific | Cat# R79007 |
| Experimental Models: Organisms/Strains | ||
| Mouse TBR1 conditional mutant | This paper | N/A |
| Mouse TBR1 constitutive mutant | This paper | N/A |
| Oligonucleotides | ||
| Primer for genotyping flox allele | This Paper | N/A |
| ND.for GAC ACA CAC CCT TCT TCA GTT TAC AGC | ||
| Primer for genotyping flox allele | This Paper | N/A |
| ND.rev CAA GCC CGA CTG CCA ATG TTC TG | ||
| Primer for genotyping Ntsr1-cre allele | This Paper | N/A |
| Ntsr1-cre.for GAC GCC ACG CCC CCC TTA | ||
| Primer for genotyping Ntsr1-cre allele | This Paper | N/A |
| Ntsr1-cre.rev CGG CAA ACG GAC AGA AGC ATT | ||
| Primer for genotyping Rbp4-cre allele | This Paper | N/A |
| Rbp4-cre.for GGG CGG CCT CGG TCC TC | ||
| Primer for genotyping Rbp4-cre allele | This Paper | N/A |
| Rbp4-cre.rev CCC CAG AAA TGC CAG ATT ACG TAT | ||
| Primer for genotyping tdTomato allele | This Paper | N/A |
| tdTomato.for CTG TTC CTG TAC GGC ATG G | ||
| Primer for genotyping tdTomato allele | This Paper | N/A |
| tdTomato.rev GGC ATT AAA GCA GCG TAT CC | ||
| Primer for genotyping constitutive allele | This Paper | N/A |
| LN.for CAT TCA GAG CGA CGC ATC AAA GC | ||
| Primer for genotyping constitutive allele | This Paper | N/A |
| LN.rev CAA GCC CGA CTG CCA ATG TTC TG | ||
| Recombinant DNA | ||
| For complete list of recombinant DNA, please refer to Table S5. | This Paper | N/A |
| pcDNA3.1(−) | Thermofisher | Cat# V79520 |
| Software and Algorithms | ||
| ImageJ | N/A | https://imagej.nih.gov/ij/ |
| MiniAnalysis | http://www.synaptosoft.com/MiniAnalysis/ | v6.0.7 |
| GraphPad Prism | https://www.graphpad.com/scientific-software/prism/ | v7.01 |
| MATLAB | https://www.mathworks.com/products/matlab.html | v8.6.0.267246 |
| Clampex and Multiclamp | https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite | v10.2 |
| ANY-maze | https://www.stoeltingco.com/any-maze-video-tracking-software-1224.html | v5 |
| Imaris | https://imaris.oxinst.com/downloads | v9.2.1 |
| Single-cell RNA-seq code availability | This Paper | https://github.com/aseveritt/Darbandi_TBR1_L5scRNAseq |