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. Author manuscript; available in PMC: 2020 Sep 4.
Published in final edited form as: J Proteome Res. 2019 May 24;18(6):2545–2558. doi: 10.1021/acs.jproteome.9b00085

Figure 3.

Figure 3.

Multiple proteases to expand the coverage and density of DSSO XL-MS protein interaction maps. (A) Distribution of CSM score for identified cross-linked peptides from cross-linked BSA digested with trypsin only or sequential LysC and trypsin or GluC only or chymotrypsin only proteases. Cross-linked BSA peptides were spiked into non-cross-linked proteome background digested with the corresponding protease. (B) Overlap of cross-linked lysine sites identified from trypsin only or sequential LysC and trypsin or GluC only or chymotrypsin only protease digestion. (C) Visualization of BSA cross-link sites identified from trypsin only, LysC and trypsin, GluC only or chymotrypsin only protease digestion, as analyzed using CID-MS2/HCD-MS2 fragmentation. Cross-links with a CSM score above the 1% score filter are highlighted in black.