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. Author manuscript; available in PMC: 2020 Sep 4.
Published in final edited form as: J Proteome Res. 2019 May 24;18(6):2545–2558. doi: 10.1021/acs.jproteome.9b00085

Figure 4.

Figure 4.

Native chromatin protein–protein interactions identified using cross-linking mass spectrometry in situ. (A) Purified chromatin bound proteins are cross-linked by cross-linking in situ. (B) Enrichment of histone protein (H3K4me3) with absence of nuclear protein (BRG1) and cytoplasmic protein (GAPDH) from chromatin bound proteins by Western blot. (C) Cross-links identified map onto known high resolution nucleosome structure (PDB ID 3av1). (D) Map of protein–protein interactions between core histone proteins (H2A, H2B, H3, H4) colored according to CSM score. (E) Map of protein–protein interactions involving linker histone H1. Proteins known to bind DNA are marked in blue.