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. 2020 Aug 25;147(16):dev193581. doi: 10.1242/dev.193581

Fig. 1.

Fig. 1.

Knockdown of Llgl1 in cultured neonatal rat cardiomyocytes decreases YAP protein levels. (A) qRT-PCR results denoting RNA transcript levels of Llgl1, Llgl2 or Yap in isolated neonatal rat cardiomyocytes following siRNA treatments. n=3. One-way ANOVA, Tukey's multiple comparisons test showing difference compared with negative control. Error bars indicate s.e.m. (B) Western blot analysis of anti-YAP and anti-γ-tubulin staining in siRNA treated rat cardiomyocytes. Quantification of anti-YAP band signal normalized to anti-γ-tubulin signal. n=6 for negative sequence, n=3 for untreated, n=9 for Llgl1, n=6 for Llgl2 and n=6 for Yap siRNA-treated cells. One-way ANOVA, Tukey's multiple comparisons test. (C) GSEA for YAP/WWTR1/TEAD target genes using RNAseq data from siRNA-treated rat cardiomyocytes targeting Llgl1 and Llgl2 or a negative control sequence. n=3. Error bars indicate s.e.m. *P<0.05, **P<0.01, ***P<0.001.