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. 2020 Aug 27;147(16):dev191023. doi: 10.1242/dev.191023

Fig. 1.

Fig. 1.

pMN progenitors express prdm8. (A) Representative transverse sections of trunk spinal cord (dorsal up), showing prdm8 RNA (blue) and olig2:EGFP (green) expression. Developmental stages noted at the top. (B) Harmony visualization of the scRNA-seq dataset from olig2:EGFP+ spinal cord cells obtained from 24, 36 and 48 hpf Tg(olig2:EGFP) embryos. Each point represents one cell (n=6489). Colors represent sample time points. (C-F) DimPlots of selected transcripts. Cells are colored by expression level (gray is low, purple is high). prdm8 expression overlaps extensively with olig2 and sox19a, and partially with nkx2.2a. (G-J) Representative transverse trunk spinal cord sections processed for fluorescent ISH to detect olig2, prdm8 and nkx2.2a mRNA at 24 hpf (G) 36 hpf (H) 48 hpf (I) and 72 hpf (J). Arrowheads indicate prdm8+/nkx2.2a+/olig2+ cells; solid arrows denote prdm8+/nkx2.2a–/olig2+ cells; dashed arrows indicate prdm8–/nkx2.2a+/olig2+ cells; and dashed ovals outline the spinal cord. Scale bars: 10 μm.