Fig. 5.

prdm8 mutant larvae have more myelinating oligodendrocytes and a deficit of OPCs. (A,C,F) Representative images of trunk spinal cord transverse sections processed to detect Sox10 expression (blue) in combination with transgenic reporter gene expression (pink). (A,B) The number of Sox10⁺ olig2:EGFP⁺ oligodendrocyte lineage cells is similar in 72 hpf wild-type (WT) and prdm8^co49 mutant larvae. Arrowheads indicate oligodendrocyte lineage cells. n=10 for both genotypes. (C-E) prdm8^co49 mutant larvae at 5 dpf have more Sox10⁺ mbpa:tagRFP⁺ oligodendrocytes (arrowheads) than wild-type larvae but there was no difference in total Sox10⁺ oligodendrocyte lineage cells. n=14 for both genotypes. (F-H) prdm8^co49 mutant larvae at 5 dpf have fewer Sox10⁺ cspg4:mCherry⁺ OPCs (arrowheads) than wild-type larvae (G), but the number of total Sox10⁺ oligodendrocyte lineage cells was unchanged (H). n=14 for both genotypes. Data are mean±s.e.m. with individual data points indicated. Statistical significance was evaluated by an unpaired, two-tailed Student's t test (B,D,E) and Mann–Whitney U test (G,H). Scale bars: 10 μm.