Fig. 6.

prdm8 mutant embryos have a deficit of motor neurons. (A,C,E) Representative images of trunk spinal cord transverse sections processed to detect Isl expression (pink) in combination with olig2:EGFP (blue). (A,B) The number of Isl⁺ olig2:EGFP⁺ motor neurons is similar in 24 hpf wild-type (WT) (n=8) and prdm8^co49 mutant (n=9) embryos. (C,D) prdm8^co49 mutant embryos at 36 hpf (n=10) have fewer Isl⁺ olig2:EGFP⁺ motor neurons than wild-type embryos (n=8). (E,F) prdm8^co49 mutant embryos at 48 hpf (n=9, 16.4±0.46) have fewer Isl⁺ olig2:EGFP⁺ motor neurons than wild-type embryos (n=8, 21.8±0.86). Data are mean±s.e.m. with individual data points indicated. Statistical significance was evaluated by an unpaired, two-tailed Student's t-test (B,F) and Mann–Whitney U test (D). Scale bars: 10 μm.