Fig. 7.

prdm8 mutant embryos prematurely switch from motor neuron to OPC production. (A) Schematic of BrdU pulses. (B-G) Representative images of trunk spinal cord sections from 48 hpf embryos treated with BrdU and processed to detect Isl (pink), Sox10 (yellow) and BrdU (blue). Wild-type (B) and prdm8^co49−/− (E) embryos pulsed with BrdU at 24 hpf. Wild-type (C) and prdm8^co49−/− (F) embryos pulsed with BrdU at 30 hpf. Wild-type (D) and prdm8^co49−/− (G) embryos pulsed with BrdU at 36 hpf. (H) Quantification of Isl⁺/BrdU⁺ motor neurons pulsed with BrdU at 24 hpf in wild type (n=15) and prdm8^co49−/− (n=7); 30 hpf in wild type (n=7) and prdm8^co49−/− (n=7); and 36 hpf in wild type (n=7) and prdm8^co49−/− (n=6). (I) Quantification of Sox10⁺/BrdU⁺ cells pulsed with BrdU at 24 hpf in wild type (n=15) and prdm8^co49−/− (n=7); 30 hpf in wild type (n=7) and prdm8^co49−/− (n=8); and 36 hpf in wild-type (n=6) and prdm8^co49−/− (n=5). Data are mean±s.e.m. with individual data points indicated. Statistical significance was evaluated by Mann-Whitney U test. *P<0.05, **P<0.001. Analysis of embryos pulsed with BrdU at 24 hpf represent data collected from two laboratory replicates. Scale bars: 10 μm.