Skip to main content
. 2020 Aug 27;147(16):dev191023. doi: 10.1242/dev.191023

Fig. 9.

Fig. 9.

Spinal cord cells of prdm8 mutant embryos have elevated Shh signaling activity. (A) Representative transverse sections of trunk spinal cords obtained from 24 and 48 hpf wild-type (WT) and prdm8co49−/− embryos (dorsal up) showing ptch2 RNA expression. (B-E) Representative transverse trunk spinal cord sections processed for fluorescent ISH to detect olig2 (blue) and ptch2 (pink) mRNA at 24 hpf (B,C) and 36 hpf (D,E). (F) prdm8co49−/− embryos have more ptch2 puncta per AU2 of spinal cord at 24 hpf (n=6) and 36 hpf (n=10) than wild-type embryos at 24 hpf (n=9) and 36 hpf (n=6). (G) Representative transverse sections of trunk spinal cord (dorsal up) showing shha RNA expression in 24 hpf wild-type and prdm8co49−/− embryos. (H) Representative transverse trunk spinal cord sections processed for fluorescent ISH to detect boc1 (blue) mRNA at 24 hpf. (I) Wild-type embryos (n=6) have more boc1 puncta per AU2 of spinal cord at 24 hpf than prdm8co49−/− embryos (n=8). Data are mean±s.e.m. with individual data points indicated. Statistical significance was evaluated by an unpaired, two-tailed Student's t-test (F) and by Mann-Whitney U test (I). *P<0.001. Dashed oval outlines the spinal cord boundary. Scale bars: 10 μm.