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. 2020 Aug 25;133(16):jcs249201. doi: 10.1242/jcs.249201

Fig. 7.

Fig. 7.

Retromer-deficient cells show reduced sensitivity to the platinum-based drug cisplatin. (A) Control and VPS35 KO HeLa cells were incubated with nine concentrations of cisplatin (from 0.39 μM to 100 μM) for 48 h and MTT reduction was measured by absorbance at 595 nm. Each data point represents the mean±s.e.m. absorbance at 595 nm normalised to the baseline reading without addition of cisplatin; n=3 independent experiments. (B) CTR1 cell surface levels were assessed by surface biotinylation in VPS35 KO H1975 cells. Cells were surface biotinylated and streptavidin–agarose used to capture biotinylated membrane proteins. The surface abundance of CTR1, ATP7A and the loading controls N-cadherin and transferrin receptor (TfR) were examined by immunoblotting. The quantification shows the mean±s.e.m.; n=3 independent experiments. (C) Control and VPS35 KO H1975 cells were incubated with nine concentrations of cisplatin (from 0.39 μM to 100 μM) for 48 h and MTT reduction was measure as described above; n=4 independent experiments. *P<0.05; **P<0.01 (unpaired Student's t-test).