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. 2020 Aug 7;57(4):967–979. doi: 10.3892/ijo.2020.5106

Figure 3.

Figure 3

LUCAT1 directly binds to miR-514a-3p and negatively alters the expression of miR-514a-3p. (A) The predicted binding sites between LUCAT1 and miR-514a-3p are presented. (B) Dual-luciferase reporter assay was performed to detect the luciferase activity in LUCAT1-WT or LUCAT1-MUT and miR-NC or miR-514a-3p co-transfected A549 and A549/DDP cells. (C) The enrichment of LUCAT1 and miR-514a-3p was analyzed by RT-qPCR after RIP assay in A549 and A549/DDP cells. (D) The level of miR-514a-3p in IMR90, A549 and A549/DDP cells was measured by RT-qPCR. (E) A549 cells were transfected with Vector or LUCAT1, A549/DDP cells were transfected with sh-NC or sh-LUCAT1, and untransfected A549 cells and A549/DDP cells were used as the control groups. The expression of miR-514a-3p was then examined by RT-qPCR. *P<0.05 vs. respective control. LUCAT1, lung cancer-associated transcript 1; DDP, cisplatin; NSCLC, non-small cell lung cancer.