Figure 5.

miR-514a-3p directly targets ULK1 to modulate ULK1 expression. (A) The potential binding sites between miR-514a-3p and ULK1 were predicted by StarBase 3.0. (B) Dual-luciferase reporter assay was conducted to confirm the association between miR-514a-3p and ULK1. (C) The enrichment of ULK1 and miR-514a-3p was analyzed by RT-qPCR following RIP assay. (D) The protein level of ULK1 in A549 cells transfected with Anti-NC or Anti-miR-514a-3p and in A549/DDP cells transfected with miR-NC or miR-514a-3p was determined by western blot analysis. (E) The protein level of ULK1 in IMR90, A549 and A549/DDP cells was detected by western blot analysis. ^*P<0.05 vs. the respective control. ULK1, uncoordinated-51-like kinase 1; DDP, cisplatin.