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. 2020 Aug 7;57(4):929–938. doi: 10.3892/ijo.2020.5107

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Copyright: © Fan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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RSV induces the apoptosis of A549 cells. A549 cells were treated with various concentrations of RSV for 48 h and (A) the proportion of apoptotic cells were detected using Annexin V-FITC/PI double staining; (B) the percentage of apoptotic cells was assessed using TUNEL staining; (C) the mRNA expression levels of Bax and Bcl-2 were detected; and (D) the protein expression levels of cleaved caspase-3/pro-caspase-3, Bax and Bcl-2 were detected. The Student's t-test and one-way ANOVA followed by a Dunnett's multiple comparison t-test were used to compare the data. ^*P<0.05, ^**P<0.01 vs. 0 µM. RSV, resveratrol; FITC, fluorescein isothiocyanate; PI, propidium iodide; TUNEL, TdT-mediated dUTP nick-end labeling.