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. 2020 Aug 11;9:e56416. doi: 10.7554/eLife.56416

Figure 6. Quantification of western blots of 3×HATbAQP2.

Cells were induced for expression of 3×HATbAQP2, pretreated with cyclohexidine and subsequently with 25 nM pentamidine (5× EC50). Western blots (Figure 6—figure supplement 1) were performed using anti-HA antiserum in order to quantify the relative amount of TbAQP2 in the cells. The two datasets were not significantly different by Kolmogorov-Smirnov test (p=0.77) and data points at each time point were also not significantly different by Student’s t-test (p>0.05). All data for these graphs are contained in Figure 6—source data 1.

Figure 6—source data 1. Densitometry readings with Western blotting of TbAQP2 turnover, normalised to 0 h.

Figure 6.

Figure 6—figure supplement 1. Western blots for a 3xHAAQP2 turnover assay in untreated T. brucei 2T1 cells, or in the presence of pentamidine 25 nM.

Figure 6—figure supplement 1.

Two independent experiments are shown. HA, haemagglutinin; tub, β-tubulin; CHX, cycloheximide; α-HA, anti-HA serum. All data for these graphs are contained in Figure 6—source data 1.