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. Author manuscript; available in PMC: 2021 Aug 10.
Published in final edited form as: J Control Release. 2020 May 16;324:317–329. doi: 10.1016/j.jconrel.2020.05.021

Table 1.

Biophysical characterization of elastin-like polypeptides evaluated in this manuscript

ELP Amino acid sequence M.W. (kDa)b Tt
(°C)c 		Rh
(nm)d 		Rg
(nm)d 		Rg/Rh Shapee
A192 MG(VPGAG)192Y 73.6 59.9 7.5
(0.2)		 N/A N/A N/A
a-FLT3-
A192 		a-FLT3a - G(VPGAG)192Y 100.1 42.3 38.6
(0.7)		 42.6 (0.7) 1.1 extended
a

the amino acid sequence is: MEVQLVQSGAEVKKPGASVKVSCKASGYTFTSYYMHWVRQAPGQGLEWMGIINPSGGSTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGVGAHDAFDIWGQGTTVTVSSGGGGSGGGGSGGGGSDVVMTQSPLSLPVTPGEPASISCRSSQSLLHSNGNNYLDWYLQKPGQSPQLLIYLGSNRASGVPDRFSGSGSDTDFTLQISRVEAEDVGVYYCMQGTHPAISFGQGTRLEIK-LVPRGS

b

expected molecular weight based on the amino acid sequence and confirmed by SDS-PAGE (Fig. 1B)

c

transition temperature defined as the maximum first derivative of the optical density at 350 nm for 25 μM ELP in PBS (Fig. 1C)

d

values indicate the mean (standard deviation) of n=5

e

the Rg/Rh ratio of α-FLT3-A192 is consistent with a ‘rod-like’ nanoparticle of an extended aspect ratio as estimated using DLS and SEC-MALS