Figure 6.

Preimplantation development in culture of naturally fertilized oocytes from N6-XX and XO females. (A) Total numbers of zygotes at the two-cell stage, as shown in parentheses, were collected at D1.5 and cultured for 3 days. Development of embryos from XO females was delayed at D1.5 + 1 and +2 days in culture compared to those from XX females (P < 0.01 by χ²-test), but no difference was found at D1.5 + 3 days. (B) Genotyping of blastocyst embryos by FISH with X- and Y-chromosome-paint (red and green, respectively) with DAPI nuclear staining. * indicates the same position in the insert at a higher magnification (×4). Bar 50 µm.