Fig. 3.

Glucose and insulin signaling pathway in inguinal white adipose tissue (iWAT), subcutaneous white adipose tissue (sWAT), liver, and skeletal muscle in 6-mo-old Bmp3b-knockout (Bmp3b^−/−) mice. Protein expression of Akt, insulin receptor substrate 1 (IRS1), and ERK1/2 phosphorylation (p) was analyzed by Western blot in iWAT, sWAT, skeletal muscle, and liver tissues from wild-type (WT) and Bmp3b^−/− male mice at 6 mo of age (A) or WT and Bmp3b^−/− female mice at 6 mo of age (B). Total Akt, IRS1, and ERK1/2 are also shown. Levels of β-actin are shown as loading controls. Densitometric analyses of Western blots are shown at bottom (n = 4 mice). Data are expressed as means ± SE. *P < 0.05 vs. WT.