Fig. 5.

Mitochondrial respiration and mitochondrial content after 1 and 8 wk of HFD-feeding in eWAT. Maximal ADP and succinate-supported (A) and lipid-supported mitochondrial respiration at 1 wk (B), ADP and succinate-supported (C), and lipid-supported mitochondrial respiration at 8 wk (D), OXPHOS protein content at 1 (E) and 8 wk (F), and representative Western blots (G) in permeabilized eWAT (n = 5–6/experiment for respiration, n = 11–12/experiment for Western blots). CIV band was faint and unable to be reliably quantified. Open bars denote LFD-fed mice, whereas solid bars denote HFD-fed mice. Data are expressed as means ± SE. *P < 0.05, compared with LFD group. AU, arbitrary units/µg protein; CI–CV, complexes I–V of the electron transport chain; DNP, 2,4-dinitrophenol; eWAT, epididymal white adipose tissue; Glut, glutamate; G3P, glycerol-3-phosphate; JO₂, oxygen flux; OXPHOS, oxidative phosphorylation; P-CoA, palmitoyl-CoA; Pyr, pyruvate; Succ, succinate.