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Pseudomonas aeruginosa exoenzyme Y (ExoY) induces time-dependent release of cleaved caspase-7. Pulmonary microvascular endothelial cells (PMVECs) were inoculated with ExoY+ for ≤6 h. A–D: every hour, cell lysates (A and C) and culture supernatants (B and D) were collected separately and analyzed for cleaved caspase-3 (A and B) or cleaved caspase-7 (C and D). E: densitometry of cleaved caspase-7 bands is represented as fold increase compared with 1 h postinfection. MW, molecular weight.
