Fig. 9.

Addition of cytokines (i.e., cytomix, a combination of TNFα, IL1β, IFNγ, and LPS) elicited a large increase in superoxide generation of C2C12 myocytes (representative examples of MitoSOX activity in A, mean data in B; images obtained at ×20 magnification). Addition of MitoTEMPOL ablated cytomix-induced increases in MitoSOX activity. Data were obtained from n = 5 plates of cells/group. Boxes represent 1 SE; center line in the box indicates the mean, and whiskers indicate the minimum and maximum values. *Statistical significance.