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. 2020 May 27;319(2):L239–L255. doi: 10.1152/ajplung.00459.2019

Fig. 1.

Fig. 1.

RNA profiling analyses of glucocorticoid receptor (GR) gain- versus loss-of-function of fetal mouse lungs. A: principle component analysis (PCA) of mesenchyme-specific GR knockout (Col1a2Cre;Nr3c1fl/fl) and wild-type mouse lung RNA microarray data (downloaded from GSE30143) from embryonic day (E)16.5 and E18.5 mouse lungs (n = 5 biological replicates were used for each time and each condition). While mRNA profiles were similar in GR-deficient mice at E16.5, deletion of GR from lung mesenchymal cells with Col1a2Cre strongly influenced gene expression and delayed maturation at E18.5. B: volcano plot shows ~800 genes differentially expressed in Col1a2Cre;Nr3c1fl/fl versus control lungs at E18.5 (ANOVA P < 0.05 and fold change >1.5). Red color indicates the genes with increased expression by 1.5-fold and blue indicates the genes with decreased expression by 1.5-fold in GR knockout mice. NR3C1 (GR) is down by 2-fold. C: heatmap of differentially expressed genes from Col1a2Cre;Nr3c1fl/fl (n = 5 lungs, denoted by red bar on the left side of the heatmap) and wild-type (n = 5 lungs, blue bar on the left side of the heatmap) at E18.5. D: enriched bioprocess and pathways induced (red bars) or suppressed (green bars) in E18.5 Col1a2Cre;Nr3c1fl/fl lungs compared with control lungs. E: heatmap of differentially expressed genes in lung mesenchymal cells FACS sorted from E18.5 Twist2Cre;Rosa26mT/mG reporter mice whose dam was injected with 1 mg/kg dexamethasone (n = 4 lungs, orange bar, left side of heatmap) or saline (n = 2 lungs, blue bar, left side of heatmap) 24 h before harvest. Bulk RNA-seq of the isolated cells was used to identify transcripts increased (red) or decreased (green) by dexamethasone treatment. Fold change >1.5 and P < 0.05 are shown. F: enriched bioprocess and pathways increased (red) or decreased (green) by dexamethasone treatment in E18.5 Twist2Cre+ lung mesenchymal cells are shown. Enriched biological functions and pathways in GR gain- vs. loss-of-function of mouse fetal lungs were largely inversely correlated in D and F.