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. 2020 Aug 24;12:7739–7751. doi: 10.2147/CMAR.S259424

Figure 4.

Figure 4

Circ-ZNF652 regulated HCC cell proliferation, migration and invasion by directly interacting with miR-29a-3p. (A) The potential binding sites between circ-ZNF652 and miR-29a-3p were predicted by starBase. (B) The luciferase activity in 293T cells co-transfected with WT-circ-ZNF652 or MUT-circ-ZNF652 and miRNA NC or miR-29a-3p mimic was determined by dual-luciferase reporter assay. (C and D) The enrichment of circ-ZNF652 and miR-29a-3p in Anti-Ago2 or anti-IgG immunoprecipitates in SNU-387 and Huh7 cells after RIP assay was measured by qRT-PCR. (E and F) MiR-29a-3p expression in SNU-387 and Huh7 cells transfected with si-NC, si-circ-ZNF652, inhibitor NC or miR-29a-3p inhibitor was examined using qRT-PCR. (G and H) Cell proliferation, (I) migration and (J) invasion in SNU-387 and Huh7 cells transfected with si-NC, si-circ-ZNF652, si-circ-ZNF652+inhibitor NC or si-circ-ZNF652+miR-29a-3p inhibitor were analyzed by MTT assay and transwell assay, respectively. *P<0.05.