Table 1.
Characteristics of different protein types encoded by AhGPAT9 in peanut germplasm.
| Protein type | Non-synonymous mutations | Amino acid differences | G3P binding sites | Catalytic centers |
|---|---|---|---|---|
| AP1 | – | – | Met256, Phe257, Lys259, Ile323, Ala326, Glu327, Arg330 | His171, Asp176 |
| AP2 | Ag.4941 T > C | p.F365S | – | His171, Asp176 |
| AP3 | Ag.1789G > A | p.S127N | Phe115, Ile116, His119, Leu121, Glu206 | His171, Asp176 |
| AP4 | Ag.2874G > A Ag.3035A > C | p.R162Q· p.T184P· | Gly56, Ala57, Asp60, Ser62, Glu206, Ala261, Phe262, Glu327, Arg330, Glu331, Ser334 | His171, Asp176 |
| AP5 | Ag.44A > G | p.E15G | Met256, Phe257, Lys259, Ile323, Ala326, Glu327, Arg330 | His171, Asp176 |
| AP6 | Ag.4595G > T | p.G346* | Met256, Phe257, Lys259, Ile323, Ala326, Glu327, Arg330 | His171, Asp176 |
| AP7 | Ag.4947A > G Ag.4950C > T | p.E367G p.S368F | – | – |
| AP8 | Ag.38A > G | p.E13G | Met256, Phe257, Lys259, Ile323, Ala326, Glu327, Arg330 | His171, Asp176 |
| AP9 | Ag.1720 T > C Ag.1798A > G | p.L104S p.K130R | Met256, Phe257, Lys259, Ile323, Ala326, Glu327, Arg330 | His171, Asp176 |
| AP10 | Ag.38A > G Ag.1720 T > C Ag.1798A > G | p.E13G p.L104S p.K130R | Met256, Phe257, Lys259, Ile323, Ala326, Glu327, Arg330 | His171, Asp176 |
| AP11 | Ag.2804A > G | p.M139V | Gly56, Ala57, Asp60, Ser62, Glu206, Met256, Phe257, Lys259, Glu327, Arg330, Glu331, Ser334 | His171, Asp176 |
| AP12 | Ag.883G > A | p.V95I | Val118, His119, Leu122, Ile204, Met256, Phe257, Ile323, Ala326, Glu327, Arg330 | His171, Asp176 |
| BP1 | – | – | Val118, His119, Leu122, Ile204, Met256, Phe257, Ile323, Ala326, Glu327, Arg330 | His171, Asp176 |
| BP2 | Bg.4328 T > C Bg.4344 T > C | p.L291F p.L296P | Met256, Phe257, Lys259, Ile323, Ala326, Glu327, Arg330 | His171, Asp176 |
| BP3 | Bg.4323A > G | p.Q289R | Met256, Phe257, Lys259, Glu327, Arg330, Glu331, Ser334 | His171, Asp176 |
| BP4 | Bg.4344 T > C | p.L296P | Met256, Phe257, Lys259, Ile323, Ala326, Glu327, Arg330 | His171, Asp176 |
| BP5 | Bg.4193A > G | p.N276S | – | – |
| BP6 | Bg.1747G > A | p.A114T | – | – |
| BP7 | Bg.1733 T > C | p.M109T | – | His171, Asp176 |
Protein types AP1 to AP12 were obtained from AhGPAT9A, while BP1 to BP7 were obtained from AhGPAT9B, and their amino acid differences compared with AP1 and BP1, respectively, were determined. p.F365S indicates a change from phenylalanine to serine at amino acid no.365 in AP2. · indicates that the amino acid changes were located in the acyltransferase domain. * indicates that the amino acid was terminal. The amino acids Phe, Glu and Arg in bold format represent typical G3P binding sites in GPAT9 proteins.