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. 2020 Sep 4;11:4418. doi: 10.1038/s41467-020-18277-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Overview of SNP-marker (red) distribution on chromosome 4 in Col-0 (green), rknob-1 and Ler-1 (gray) spanning the inversion and both border regions. Two SNP-markers form one interval resulting in six different intervals (I1-I6). b Results of recombination analysis based on SNP genotyping of pollen nuclei in F1-hybrids. Fifty-two samples were used for rknob-1 × Ler-1 and 90 samples for the control. Recombination frequencies are given as cM/Mb, with the amount of CO being set in relation to the distance between the two corresponding markers. The inverted area is highlighted in light blue in the diagram. c Results of recombination analysis based on SNP genotyping of leaf material of F2-hybrids. 198 samples were analyzed for rknob-1 × Ler-1 and 200 samples for the control. Recombination frequencies are given as cM/Mb, with the amount of CO being set in relation to the distance between the two corresponding markers. The inverted area is highlighted in light blue in the diagram. d Detailed overview of composition and amount of detected CO and no recombination (NR) events in haploid pollen tissue (F1) and diploid leaf tissue (F2) in the rknob-1 × Ler-1 line. Detection of Col-0 allele is marked in light green, detection of Ler-1 allele is marked in gray, and when both alleles were detected the marker is shown in dark green. The inverted area is highlighted in light blue. Source data are provided as a Source Data file.