Table 5.

Matrix effects and extraction recoveries for the analytes and three internal standards in rat plasma (mean ± SD, n = 5).

Analytes	Spiked concentration (ng/mL)	Matrix effect (%)		Recovery (%)
		Mean ± SD	RSD%	Mean ± SD	RSD%
Coclaurine	0.16	103.36 ± 0.03	2.98	94.81 ± 0.05	12.33
	1.60	98.91 ± 0.03	2.90	96.91 ± 0.04	6.98
	12.80	103.15 ± 0.05	5.10	95.83 ± 0.03	3.29
Magnoflorine	9.04	103.67 ± 0.04	3.81	96.24 ± 0.03	14.50
	45.20	102.70 ± 0.04	3.87	98.78 ± 0.02	2.20
	361.60	102.01 ± 0.05	4.80	96.40 ± 0.02	2.26
Spinosin	6.00	107.70 ± 0.08	7.64	84.91 ± 0.08	9.76
	24.00	104.11 ± 0.11	10.99	87.28 ± 0.03	3.03
	192.00	98.86 ± 0.01	0.86	94.75 ± 0.02	2.01
6‴-Feruloylspinosin	0.40	111.26 ± 0.06	4.24	92.38 ± 0.08	8.55
	2.00	87.45 ± 0.04	4.32	95.76 ± 0.03	2.89
	16.00	112.28 ± 0.02	1.73	96.09 ± 0.08	7.02
Jujuboside A	1.64	95.81 ± 0.09	9.67	90.14 ± 0.11	11.82
	6.56	92.09 ± 0.11	11.64	83.48 ± 0.06	6.73
	52.48	97.73 ± 0.05	5.06	93.01 ± 0.08	8.11
Jujuboside B	1.06	90.76 ± 0.02	2.14	97.25 ± 0.09	9.50
	4.24	95.67 ± 0.09	9.49	91.80 ± 0.12	12.98
	33.92	97.43 ± 0.04	4.20	98.92 ± 0.04	3.55
IS1	7.87	106.00 ± 0.08	7.43	92.12 ± 0.07	7.95
IS2	21.60	98.00 ± 0.05	5.30	95.35 ± 0.05	4.81
IS3	55.68	109.69 ± 0.03	3.07	95.84 ± 0.04	3.85