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. 2020 May 4;10(3):658–671.e1. doi: 10.1016/j.jcmgh.2020.04.015

Figure 8.

Figure 8

Effects of NE on membrane potentials of human S colon circular SMCs. Application of NE 10 μmol/L induced a two-phase hyperpolarization, a rapid component followed by a sustained component. (A) NE-induced two-phase hyperpolarization was changed to small, sustained hyperpolarization by pretreatment of prazosin (Praz) 1 μmol/L. Residual hyperpolarization was inhibited by propranolol (Pro) 10 μmol/L. (B) Summarized bar graphs showing effects of Praz and Pro on NE-induced two-phase hyperpolarization. ∗∗P < .01, significant difference from control responses of rapid component. †P < .05, significant difference from control responses of sustained component. ††P < .01, significant difference from sustained responses in presence of Praz alone. (C) Apamin (Apa) 0.1 μmol/L inhibited two-phase hyperpolarization induced by NE, resulting in sustained hyperpolarization, which was inhibited by Pro 10 μmol/L. (D) Summary showing effects of Apa and Pro on NE-induced two-phase hyperpolarization. ∗∗P < .01, significant difference from control responses of rapid component. †P < .05, significant difference from control responses of sustained component. ††P < .01, significant difference from sustained responses in presence of Apa alone. Resting membrane potentials were A, −46 mV; C, −49 mV. A and C were recorded from different tissues. Each record in a given set of two was obtained from the same impalement.