Figure 2.

Hybridization kinetic curves measured by RPI. Binding curves (black dots) are expressed as mass surface density measured after the injection of 100 nM of target DNA in solution with 150 mM NaCl. The different curves refer to spots on the same RPI sensor with different surface density σ_p of DNA probes (no linker type), as reported in the figure. The DNA probes are immobilized via MCP2 copolymer. The continuous lines represent the fits with single exponential growth functions with initial slope constrained to that of the data points (blue) and numerical solutions of Eq. 6 (red). To see this figure in color, go online.